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Phusion Dna Polymerase Pdf Free

 

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Phusion Dna Polymerase Pdf Free, download facebook chat for lg a165

 

Monarch kits are available for plasmid miniprep, gel extraction and reaction cleanup. Due to the increased processivity of Phusion DNA Polymerase there is no advantage in increasing dNTP concentrations. Conversely, inadequate Mg2 could lead to lower product yield. Our unique column design offers elution in lower volumes and eliminates buffer retention, resulting in high quality DNA for downstream applications. Our unique column design offers elution in lower volumes and eliminates buffer retention, resulting in high quality DNA for downstream applications. Customer Service 1-800-632-5227 FAX: 1-800-632-7440 Monday - Friday 9:00 AM - 8:00 PM EST customerserviceneb.com New England Biolabs Inc. Sign in Invalid username and/or password. Excess mg2 can also stabilize spurious annealing of primers to incorrect template sites and decrease specificity. Following the guidelines will ensure optimal enzyme performance. Documents Manuals & protocols Recommended products Phusion Green High-Fidelity DNA Polymerase (2 U/L) Catalog number: F534S Size 100 units Price EUR 97,00 Qty Add to favorites Phusion High-Fidelity PCR Master Mix with HF Buffer Catalog number: F531S Size 100 reactions Price EUR 165,00 Qty Add to favorites dNTP Mix (10 mM each) Catalog number: R0191 Size 0.2 mL Price EUR 23,57 Qty Add to favorites . Usually 1 unit of Phusion DNA Polymerase per 50 μl reaction volume gives good results, but optimal amounts could range from 0.5-2 units per 50 μl reaction depending on amplicon length and difficulty. Customized Solutions & OEM NEBsolutionsneb.comInquiry Form New England Biolabs Inc. .. Phusion DNA Polymerase tends to work better at elevated denaturation and annealing temperatures due to higher salt concentrations in its buffer. Pfu DNA polymerase is an enzyme found in the hyperthermophilic archaeon Pyrococcus furiosus, where it functions to copy the organism's DNA during cell division. If you continue without changing your settings, we'll assume that you are happy to receive all cookies on Thermo Fisher Scientific websites. Phusion DNA Polymerases offer robust performance with short protocol times, even in the presence of PCR inhibitors, and generate higher yields with lower enzyme amounts than other DNA polymerase.Highlights� High fidelity (52X Taq)� Fast PCR due to short extension times (15-30 s/kb)� Robust performance, minimal optimization needed� High yields of PCR products with minimal enzyme amounts� Available as a Green buffer format for direct loading of PCR products on gels (F-534S or F-534L)Applications� High-fidelity PCR� Cloning� Template generation for sequencing� Amplification of difficult (GC-rich) templates� Long-range PCR (up to 20 kb)� Mutagenesis� High throughput PCR� Microarray For Research Use Only. Monarch kits are available for plasmid miniprep, gel extraction and reaction cleanup. For some cDNA templates, the extension time can be increased up to 40 seconds per 1 kb to obtain optimal results. Not for use in diagnostic procedures. For further optimization DMSO should be varied in 2% increments. My NEB Products Save your favorite products by clicking Add to My NEB, making re-ordering and remembering what you need quick and simple. Phusion High-Fidelity DNA Polymerase (2 U/L) Catalog number:F530S Thermo Scientific� Related applications: PCR Contact us for support � Error loading your content! Catalog number Select a plan Unit size Price({{currency}}) Your price({{currency}}) Availability Qty {{product.sku}} {{product.sku}} also known as {{product.formattedSku}} {{plan.description}} {{subscriptionDetails.selectedRatePlan.billingPeriod}} {{subscriptionDetails.selectedRatePlan.billingPeriod}} {{product.availability.message}} � Pro add-ons {{currency}} Check your price & availability � Check your availability � Check your price & availability � Check your availability � Add To Cart Customize this product� Need a web quote?� Check your on-site stock� Your on-site stock �� {{supplyCenter.scName}}({{scProduct.stockOnHand}} In stock) �� {{supplyCenter.scName}}(Out of stock) �� {{supplyCenter.scName}} This item is not currently available on-site. 1991 Dec 12;108(1):1-6). Using Pfu DNA polymerase in PCR reactions also results in blunt-ended PCR products. 6704223018

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